NEXCOPE NIB1000

SCIENTIFIC RESEARCH CLASS FULLY AUTOMATIC INVERTED MICROSCOPE

NEXCOPE NIB1000 Open platform for all microscopy applications

Designed with high-speed, fully motorized automation, this system is compatible with the Adaptive Focusing System (AFS), making it an ideal choice for high-level cellular research. Its dual-layer optical path enables limitless expansion, offering a powerful, flexible, and future-ready imaging platform for live-cell, confocal, and super-resolution microscopy.

Robust and Scalable Live-Cell Imaging Platform

The NIB1000 delivers an advanced imaging platform with a 25 mm field of view, designed to meet the demands of experiments requiring wide-area observation. Fully motorized, the NIB1000 integrates multiple imaging modes including brightfield, fluorescence, DIC, and phase contrast. Users can choose between single- or dual-layer optical paths, and the optional anti-drift focusing module makes it especially well-suited for long-term, dynamic live-cell imaging.

Offering a stable and reliable foundation for advanced microscopy techniques such as confocal, super-resolution, and single-molecule imaging, the NIB1000 is built for high-end research environments. A responsive touchscreen interface records sensor status and user habits, allowing seamless one-touch switching between application modes and smooth control of motorized components.

Combined with powerful acquisition and analysis software, the NIB1000 stands out as a scalable and dependable system—ready to meet diverse experimental needs while delivering accurate, repeatable results.

25mm Wide Field of View

With the growing demand for large-scale, high-throughput, and intelligent research solutions, faster data acquisition and higher efficiency have become essential. The NIB1000's wide-field illumination and fluorescence architecture deliver uniform, high-brightness imaging for both brightfield and fluorescence modes.

Its large sensor and imaging interface are designed to fully maximize the performance of large-format detectors, offering an ideal imaging platform built for the future of rapidly advancing camera technologies.

Wide-Area Illumination Module

Bright and Wide-Field Transmitted Illumination

The transmitted illumination module adopts high-power LED lights to ensure full-field brightness and uniform illumination. This provides clear and consistent results, especially in high-magnification DIC applications, while delivering a stable light source ideal for seamless image stitching.

Large-Diameter Fluorescence Reflector Illuminator

Specifically designed for FOV 25mm fluorescence imaging, this compact fiber-optic illuminator is equipped with a high-power LED light box that delivers high-transmission illumination across a broad spectrum—including ultraviolet light. It is fully compatible with large-diameter fluorescence filters, enabling high signal-to-noise ratio fluorescence images over the full 25mm field of view.

Large-Diameter Photoscopic Optical System

Equipped with a large-diameter tube lens designed to expand the optical path and work seamlessly with wide-target CMOS sensors, the NIB1000 achieves a 25mm field of view (FOV) for both brightfield and fluorescence imaging.

FOV 25mm Objectives

Objectives with exceptional image flatness deliver high-quality imaging. They fully maximize the potential of the FOV 25 objective lenses, significantly increasing data acquisition speed.

Ideal Live-Cell Imaging Platform

Even minor changes in temperature and vibration within the imaging environment can significantly impact focus stability. The Adaptive Focus Shift Prevention System (AFS) eliminates focus drift through both static and dynamic measurements, enabling time-lapse observation of live cells over several days or longer with a high-brightness LED fluorescence light source and cell culture system. The NIB1000 is the perfect imaging tool for demanding and intensive experiments.

Cell Culture System

Designed specifically for live-cell imaging, the cell culture system provides a stable environment for live-cell experiments by precisely controlling the microscope platform’s temperature while maintaining the culture chamber’s internal temperature, humidity, and CO2 concentration

Stable and Reliable Imaging with AFS

Focus stability is a critical factor in live-cell imaging. Even in controlled temperature and humidity environments, slight shifts in the microscope’s focus position can occur due to temperature fluctuations and vibrations, significantly impacting long-term live-cell observations. The NIB1000 adopts an independent focus design to minimize the effect of other mechanical components on the Z-axis. Its fully redesigned Adaptive Focus System (AFS) features an adaptive anti-focus drift mechanism that eliminates focus shift. You can maintain sharp focus at all times, even when using high-magnification, high-NA objectives and advanced imaging technologies such as super-resolution, confocal, and TIRF.

Advanced LED Fluorescence Illumination System

ED 4 supports up to 4-channel LED illumination and is compatible with commonly used fluorescent dyes, focusing on excitation wavelengths. It offers high brightness to meet the needs of routine experimental fluorescence imaging. The long-lasting LEDs require no replacement. Compared to traditional mercury lamps, it provides low photobleaching and phototoxicity, making it ideal for live-cell samples.

Sustainable, energy-efficient, and with a low carbon footprint, it is an environmentally friendly ideal microscope light source.

Intuitive and Ergonomic Controls

The NIB1000 combines a range of interactive technologies to provide precise control over imaging. Its intuitive and user-friendly microscope operation interface simplifies complex procedures, enabling researchers to work more efficiently and comfortably. This helps reduce user fatigue and minimizes damage to live cells.Etkiletişimli Manipülasyon

The NIB1000’s touch front panel enhances the interaction between the user and the microscope, making it more intuitive, convenient, and expandable. Buttons located on the left and right sides provide easy control even in dark environments, allowing researchers to focus more on their experiments.

The 5.6-inch touchscreen on the front controls the movements of electric components such as the objective turret, dual/single fluorescence filter wheel, condenser, light source, motorized XY stage, Z-axis speed, light path, ESC escape, FN key, and objective focusing.

The real-time display shows objective magnification, transmitted light brightness, fluorescence channels, output ports, and XYZ position and speed status.

High-Speed Motorized Control

Objective lens, filter, XY stage, and observation module operation and switching speeds have been significantly improved, providing researchers with a seamless workflow to focus on repetitive observations and imaging. Additionally, the joystick allows free control of the stage, designed to operate the microscope as naturally as the user’s eyes and hands..

Joystick with Built-in Touch Panel

Equipped with a touchscreen for adjusting the main body, the joystick offers speed control for the motorized XY stage, motorized focus adjustment, FN function keys, and AFS focus stabilization control.

Excellent Optical System

The NIB1000’s high-quality infinity-corrected optical system supports brightfield, fluorescence, phase contrast, DIC, Hoffman, and other advanced observation methods. Regardless of the imaging technique, it delivers bright, original images with a high signal-to-noise ratio. Researchers highly appreciate its exceptional optical performance and stable reliability.

Phase Contrast

The condenser features an internal phase contrast ring that allows smooth switching from brightfield observation to phase contrast by rotating the condenser turret. The semi-apochromatic phase contrast objective lens delivers sharp images with high contrast.

Floresans

The newly designed filter set achieves an OD6 blocking rate in a single-channel fluorescence filter, delivering high signal-to-noise ratio images suitable for single-molecule and single-cell experiments. The light module's spectral design covers a wide wavelength range, while the six-position rotary filter turret meets diverse fluorescence requirements. Additionally, the high-power LED fiber-optic light source provides bright and safe illumination.

DIC

The high-quality DIC optical system with a wide aperture delivers smooth, sharp, and detailed images with high resolution and contrast for every sample at all magnification levels.

Hoffmann

High-contrast phase contrast imaging technique for transparent, unstained samples such as eggs. The Hoffman phase contrast component provides 3D-like images appearing as projection shadows. Contrast direction can be easily adjusted for each individual sample.

NEXCOPE NOMIS PRO X Microscope Control and Analysis

The NIB1000’s Nomis Pro X software platform integrates hardware control, image acquisition, and processing into a single application. Nomis Pro X makes microscope operation highly versatile and user-friendly, even for complex imaging tasks. It also enables fast and easy hardware and software connectivity.

Image processing capabilities include measurement and adjustment, filtering, noise reduction, deconvolution, and SIM reconstruction—designed to meet the needs of diverse working environments.

Multidimensional Imaging

You can configure and save settings to meet various imaging needs such as multi-channel, multi-position, time-lapse imaging, or large image stitching.

Multidimensional Imaging Display

Visualize multi-channel, time-lapse, Z-stack, XY positioning, and image stitching information in a single window, enhancing the review and analysis of experimental results.

High-Speed Hardware Control

The software controls various electronic microscope components, including objective lens changes, focus adjustments, condenser changes, and fluorescence module switching.

Deconvolution

It can correct blurring in 2D images. The process is reusable, and results can be adjusted on-screen. 3D deconvolution is also applicable for multidimensional imaging.

Exceptional System Scalability

Adaptive Focus Shift Prevention System (AFS)

Automatically compensates for focus drift caused by temperature changes and mechanical vibrations.

Stage Position Shifting Device

A second fluorescence illumination module is available beneath the stage.